Inactivation of the ATP-dependent DNase of Escherichia coli after infection with double-stranded DNA phages.
نویسنده
چکیده
The ATP-dependent DNase activity of Escherichia coli disappeared or was markedly reduced after infection with double-stranded DNA phages, T2, T3, T4, T5, T6, T7, lambda, phi80, and P1, but not with the single-stranded DNA phage f1, or the RNA phage Qbeta. This DNase activity was not reduced when chloramphenicol was added prior to phage infection.
منابع مشابه
Destruction of biological particles using non-thermal plasma
Mechanism of inactivation of bio-particles exposed to non-thermal plasma (NTP), namely, dielectric barrier discharge (DBD), and plasma jet (PJ), has been studied using E. coli, B. subtilis spore, S. cerevisiae and bacteriophages. States of different biological components were monitored during the course of inactivation. Analysis of green fluorescent protein, GFP, introduced into E. coli. or B. ...
متن کاملSelective inhibition of the dnase activity of the recBC enzyme by the DNA binding protein from Escherichia coli.
In the presence of the Escherichia coli DNA binding protein, single-stranded DNA is resistant to both the endo- and exonucleolytic activities of the recBC DNase. Linear duplex DNA, on the other hand, is unwound at a normal rate, but converted to large, single-stranded fragments which are resistant to further hydrolysis. Therefore, in the presence of the binding protein and linear duplex DNA, th...
متن کاملSelective Inhibition of the DNase Activity of the recBC Enzyme by the DNA Binding Protein from Escherichia COG*
In the pesence of the Escherichia coli DNA binding protein, single-stranded DNA is resistant to both the endoand exonucleolytic activities of the recBC DNase. Linear duplex DNA, on the other hand, is unwound at a normal rate, but converted to large, single-stranded fragments which are resistant to further hydrolysis. Therefore, in the presence of the binding protein and linear duplex DNA, the r...
متن کاملMolecular Effects of Atmospheric Pressure Plasma Jet on the Double-Stranded DNA
Introduction The aim of this study was toinvestigate the sterilization potential of atmospheric pressure plasma jet (APPJ) and interactions of this technology with double-stranded DNA using the polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) techniques. Materials and Methods The plasma jet was produced through a high voltage sinusoidal power supplyusing a mixt...
متن کاملSelective Inhibition of the DNase Activity of the recBC Enzyme by the DNA Binding Protein from Escherichia COG*
In the pesence of the Escherichia coli DNA binding protein, single-stranded DNA is resistant to both the endoand exonucleolytic activities of the recBC DNase. Linear duplex DNA, on the other hand, is unwound at a normal rate, but converted to large, single-stranded fragments which are resistant to further hydrolysis. Therefore, in the presence of the binding protein and linear duplex DNA, the r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of virology
دوره 14 6 شماره
صفحات -
تاریخ انتشار 1974